Saponification Value

Request a quote

Saponification value

Saponification is a process that involves the conversion of fat, oil, or lipid, into soap and alcohol by the action of aqueous alkali.

Saponification value or saponification number (SV or SN) is defined as the amount of potassium hydroxide (KOH) in milligrams required to saponify one gram of fat or oil under the conditions specified. It is a measure of the average molecular weight (or chain length) of all the fatty acids present in the sample as triglycerides. The higher the saponification value, the lower the fatty acids average length, the lighter the mean molecular weight of triglycerides and vice-versa.

Test Method

To determine saponification value, the sample is hot-saponified with an excess of alkali (usually potassium hydroxide dissolved in ethanol), in standard conditions, generally for half an hour under reflux. At the end of the reaction the remaining quantity of alkali is titrated against standard solution of hydrochloric acid (HCl). Therefore, the SV (mg KOH/g of sample) is calculated as following:

saponification value


V1 - HCl, for blank, mL
V2 - HCl, for sample, mL
N - the molarity of HCl solution, mol · L−1
W - weight of sample (dry basis), g
56.1 - molecular weight of KOH, g · mol−1


Alfa Chemistry's Advantages

  • Short Detection Cycle
  • Advanced Equipment
  • Competitive Price
  • Reliable Results
  • Years of Testing Experience
  • Familiar with Relevant Standards

The chemists at Alfa chemistry have extensive experience in the testing of chemical substances. Alfa Chemistry strictly follows ASTM D5558, ASTM D94, ASTM D138 and other standards to provide you with a variety of chemical saponification value test. Please feel free to contact us for more details.

Our products and services are for research use only and cannot be used for any clinical purpose.

Ask Your Question