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Microbial detection is used in medical, food, brewing, chemical, petroleum, environmental protection and other fields. Microbiology test covers the use of biological, biochemical or chemical methods for the detection, identification or enumeration of microorganisms. It is often applied to disease causing and spoilage microorganisms.
- Microbial characteristics
- Small volume, large surface area
- Wide distribution and many species
- More absorption, fast transformation
- Grow prosperous, reproduce fast
- Adapt to strong, easy variation
- Microbial classification
- Prokaryotic cellular microorganism
- Acellular microorganism
- Eukaryotic cellular microorganism
- Test methods
- Microbiological count method
- Microbial mass measurement
- Microbial physiology method
Microbiological count methods are the accepted laboratory methods used to estimate the microbial population of a tested substance. The most commonly used microbiological count method is the standard plate count (SPC) agar method.
SPC is used to estimate the population density of bacteria in a broth by plating a small and dilute portion of the sample and counting the number of bacteria colonies. Microbiologists use serial dilutions to create decreasing concentrations of the original sample that are then plated so that a plate will be created with a low enough number of bacteria that we can count individual colonies. From that number, the original cell density in the broth can be calculated.
Changes in the number of microorganisms can be calculated by a variety of methods that focus on microbial mass.
There are several methods for measuring cell mass, including: i) the gravimeter method . ii) turbidimetry method. Spectrophotometers are electrical appliances that can measure turbidity very accurately (see Fig.1).
Fig. 1 Microbiology test by spectrophotometer
Culture media can be used to differentiate between different kinds of microorganisms by detecting acid or gas production.
Differential media or indicator media distinguish one microorganism type from another growing on the same media. By testing the nitrogen content, carbon content, reducing sugar content, acid generation, gas production rate, etc. we can identify the types of microorganisms and their growth state.
- Microorganism species
- Bacillus cereus
- E. coli/coliforms
- Fecal and total coliforms
- rocarbon utilizing bacteria
- HydHeterotrophic or total plate count (HPC, TPC)
- Iron related bacteria
- Lactic Acid Bacteria Count
- Pseudomonas aeruginosa
- Total Bacillus Count
- Total plate count/aerobic plate count/standard plate count
- Vibrio parahaemolyticus
- Yeast and mold
- Alfa Chemistry's advantages
- Short Detection Cycle
- Competitive Price
- Years of Testing Experience
- Advanced Equipment
- Reliable Results
- Familiar with Relevant Standards
Alfa Chemistry offers microbiology test of various chemicals. Our microbiology test serves a wide range of customers in different industries, including food, cosmetics, detergents and packaging products. We can help you evaluate the safety or efficacy of raw materials, components, ingredients and finished products. Types of tests include: